human recombinant igf 1 protein Search Results


recombinant human igf 1  (R&D Systems)
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recombinant human igf  (R&D Systems)
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igf 1  (R&D Systems)
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igf 1  (Novus Biologicals)
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Novus Biologicals igf 1
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recombinant human lr3 insulin like growth factor i  (R&D Systems)
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igf 1 lr3  (R&D Systems)
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human recombinant insulin like growth factor i igf i  (R&D Systems)
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Human Recombinant Insulin Like Growth Factor I Igf I, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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igf1  (Elabscience Biotechnology)
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Elabscience Biotechnology igf1
Figure 3 The CTCFR567W variant inhibition of IGFs gene expression. (A) qPCR analysis of the expression level of CTCF mRNA in the wild and mutant groups in three different cell lines. (B) Western blot analysis of the expression level of CTCF protein in the wildtype and mutant groups in LO2 cells. FLAG is a label protein of vectors. (C) In HEK-293T, LO2, and LCLs, mRNA levels of <t>IGF1</t> were measured using quantitative PCR. (D) IGF1 protein levels were analyzed with ELISA. (E) In HEK-293T, LO2, and LCLs, mRNA levels of IGFBP3 were measured using quantitative PCR. (D) IGFBP3 protein levels were analyzed with ELISA. For quantitative PCR, results were normalized to β-actin as a reference and compared with control cells transfected with an empty vector plasmid (n ≥ 3; mean ± s.e.m. shown; ***P < 0.001 by Student t-test). For Western blotting, results were normalized to β-actin as a reference (n = 3; mean ± s.e.m. shown; *P < 0.05, **P < 0.01, and ***P < 0.001). A full color version of this figure is available at https://doi.org/10.1530/JME-22-0193.
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human igf 1  (R&D Systems)
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R&D Systems human igf 1
Figure 3 The CTCFR567W variant inhibition of IGFs gene expression. (A) qPCR analysis of the expression level of CTCF mRNA in the wild and mutant groups in three different cell lines. (B) Western blot analysis of the expression level of CTCF protein in the wildtype and mutant groups in LO2 cells. FLAG is a label protein of vectors. (C) In HEK-293T, LO2, and LCLs, mRNA levels of <t>IGF1</t> were measured using quantitative PCR. (D) IGF1 protein levels were analyzed with ELISA. (E) In HEK-293T, LO2, and LCLs, mRNA levels of IGFBP3 were measured using quantitative PCR. (D) IGFBP3 protein levels were analyzed with ELISA. For quantitative PCR, results were normalized to β-actin as a reference and compared with control cells transfected with an empty vector plasmid (n ≥ 3; mean ± s.e.m. shown; ***P < 0.001 by Student t-test). For Western blotting, results were normalized to β-actin as a reference (n = 3; mean ± s.e.m. shown; *P < 0.05, **P < 0.01, and ***P < 0.001). A full color version of this figure is available at https://doi.org/10.1530/JME-22-0193.
Human Igf 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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egf  (Boster Bio)
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Boster Bio egf
Figure 3 The CTCFR567W variant inhibition of IGFs gene expression. (A) qPCR analysis of the expression level of CTCF mRNA in the wild and mutant groups in three different cell lines. (B) Western blot analysis of the expression level of CTCF protein in the wildtype and mutant groups in LO2 cells. FLAG is a label protein of vectors. (C) In HEK-293T, LO2, and LCLs, mRNA levels of <t>IGF1</t> were measured using quantitative PCR. (D) IGF1 protein levels were analyzed with ELISA. (E) In HEK-293T, LO2, and LCLs, mRNA levels of IGFBP3 were measured using quantitative PCR. (D) IGFBP3 protein levels were analyzed with ELISA. For quantitative PCR, results were normalized to β-actin as a reference and compared with control cells transfected with an empty vector plasmid (n ≥ 3; mean ± s.e.m. shown; ***P < 0.001 by Student t-test). For Western blotting, results were normalized to β-actin as a reference (n = 3; mean ± s.e.m. shown; *P < 0.05, **P < 0.01, and ***P < 0.001). A full color version of this figure is available at https://doi.org/10.1530/JME-22-0193.
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Image Search Results


Figure 3 The CTCFR567W variant inhibition of IGFs gene expression. (A) qPCR analysis of the expression level of CTCF mRNA in the wild and mutant groups in three different cell lines. (B) Western blot analysis of the expression level of CTCF protein in the wildtype and mutant groups in LO2 cells. FLAG is a label protein of vectors. (C) In HEK-293T, LO2, and LCLs, mRNA levels of IGF1 were measured using quantitative PCR. (D) IGF1 protein levels were analyzed with ELISA. (E) In HEK-293T, LO2, and LCLs, mRNA levels of IGFBP3 were measured using quantitative PCR. (D) IGFBP3 protein levels were analyzed with ELISA. For quantitative PCR, results were normalized to β-actin as a reference and compared with control cells transfected with an empty vector plasmid (n ≥ 3; mean ± s.e.m. shown; ***P < 0.001 by Student t-test). For Western blotting, results were normalized to β-actin as a reference (n = 3; mean ± s.e.m. shown; *P < 0.05, **P < 0.01, and ***P < 0.001). A full color version of this figure is available at https://doi.org/10.1530/JME-22-0193.

Journal: Journal of Molecular Endocrinology

Article Title: CTCF variant begets to short stature by down-regulation of IGF1

doi: 10.1530/jme-22-0193

Figure Lengend Snippet: Figure 3 The CTCFR567W variant inhibition of IGFs gene expression. (A) qPCR analysis of the expression level of CTCF mRNA in the wild and mutant groups in three different cell lines. (B) Western blot analysis of the expression level of CTCF protein in the wildtype and mutant groups in LO2 cells. FLAG is a label protein of vectors. (C) In HEK-293T, LO2, and LCLs, mRNA levels of IGF1 were measured using quantitative PCR. (D) IGF1 protein levels were analyzed with ELISA. (E) In HEK-293T, LO2, and LCLs, mRNA levels of IGFBP3 were measured using quantitative PCR. (D) IGFBP3 protein levels were analyzed with ELISA. For quantitative PCR, results were normalized to β-actin as a reference and compared with control cells transfected with an empty vector plasmid (n ≥ 3; mean ± s.e.m. shown; ***P < 0.001 by Student t-test). For Western blotting, results were normalized to β-actin as a reference (n = 3; mean ± s.e.m. shown; *P < 0.05, **P < 0.01, and ***P < 0.001). A full color version of this figure is available at https://doi.org/10.1530/JME-22-0193.

Article Snippet: The protein expression levels of IGF1 and IGFBP3 were quantified using Human IGF1 ELISA Kit (Elabscience, Wuhan, China) and Human IGFBP-3 ELISA Kit (Elabscience), respectively.

Techniques: Variant Assay, Inhibition, Gene Expression, Expressing, Mutagenesis, Western Blot, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Control, Transfection, Plasmid Preparation

Figure 4 Regulation of IGF1 expression by CTCF. (A, B) The transcriptional activity of IGF1 responds to the CTCFR567W variant by luciferase assay in HEK-293T cell. Co-transfected HEK-293T cells with wildtype or mutant CTCF expression plasmids and IGF1 reporter recombinant plasmids. (A) Luciferase activity was determined at 24, 36, 48, 60, and 72 h after transfection. (B) For comparison, the wildtype values were adjusted to 1’; a histogram represents the relative value of transcriptional activities. Data are means ± s.e.m. of three independent experiments (*P < 0.5; **P < 0.001; ***P < 0.0001; t-test). (C, D) The interaction between the CTCFR567W variant and IGF1 by CHIP-qPCR assay. (C) The diagram of full-length (FL) of IGF1 gene promoter (pmIGF1-FL). (D) CHIP-qPCR assay of the occupation rate of CTCF on the IGF1 primer region. NC, negative control; pm: promoter; TBE:,transcription binding elements; TSS, transcription start sites. Data are means ± s.e.m. of three independent experiments (*P < 0.5; **P < 0.001; ***P < 0.0001; t-test). A full color version of this figure is available at https://doi.org/10.1530/JME-22-0193.

Journal: Journal of Molecular Endocrinology

Article Title: CTCF variant begets to short stature by down-regulation of IGF1

doi: 10.1530/jme-22-0193

Figure Lengend Snippet: Figure 4 Regulation of IGF1 expression by CTCF. (A, B) The transcriptional activity of IGF1 responds to the CTCFR567W variant by luciferase assay in HEK-293T cell. Co-transfected HEK-293T cells with wildtype or mutant CTCF expression plasmids and IGF1 reporter recombinant plasmids. (A) Luciferase activity was determined at 24, 36, 48, 60, and 72 h after transfection. (B) For comparison, the wildtype values were adjusted to 1’; a histogram represents the relative value of transcriptional activities. Data are means ± s.e.m. of three independent experiments (*P < 0.5; **P < 0.001; ***P < 0.0001; t-test). (C, D) The interaction between the CTCFR567W variant and IGF1 by CHIP-qPCR assay. (C) The diagram of full-length (FL) of IGF1 gene promoter (pmIGF1-FL). (D) CHIP-qPCR assay of the occupation rate of CTCF on the IGF1 primer region. NC, negative control; pm: promoter; TBE:,transcription binding elements; TSS, transcription start sites. Data are means ± s.e.m. of three independent experiments (*P < 0.5; **P < 0.001; ***P < 0.0001; t-test). A full color version of this figure is available at https://doi.org/10.1530/JME-22-0193.

Article Snippet: The protein expression levels of IGF1 and IGFBP3 were quantified using Human IGF1 ELISA Kit (Elabscience, Wuhan, China) and Human IGFBP-3 ELISA Kit (Elabscience), respectively.

Techniques: Expressing, Activity Assay, Variant Assay, Luciferase, Transfection, Mutagenesis, Recombinant, Comparison, ChIP-qPCR, Negative Control, Binding Assay